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Literature summary extracted from

  • Wang, X.; Zhou, Y.; Wang, L.; Liu, W.; Liu, Y.; Peng, C.; Zhao, Z.
    Engineering Escherichia coli nicotinic acid mononucleotide adenylyltransferase for fully active amidated NAD biosynthesis (2017), Appl. Environ. Microbiol., 83, e00692 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.1 A86W/Y118N increase in activity with nicotinic acid ribonucleotide, and mutant accepts nicotinamide ribonucleotide as substrate Escherichia coli
2.7.7.1 Y84V/Y118D mutant prefers nicotinamide ribonucleotide over nicotinic acid ribonucleotide as substrate Escherichia coli
2.7.7.18 A86W/Y118N increase in activity, and mutant accepts nicotinamide ribonucleotide as substrate Escherichia coli
2.7.7.18 Y84V/Y118D mutant prefers nicotinamide ribonucleotide over nicotinic acid ribonucleotide bas substrate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.1 0.76
-
 nicotinamide ribonucleotide mutant A86W/Y118N, pH 7.5, 30°C Escherichia coli
2.7.7.1 0.8
-
 nicotinamide ribonucleotide mutant Y84V/Y118D, pH 7.5, 30°C Escherichia coli
2.7.7.1 9.4
-
 nicotinamide ribonucleotide wild-type, pH 7.5, 30°C Escherichia coli
2.7.7.18 0.07
-
 beta-nicotinate D-ribonucleotide mutant A86W/Y118N, pH 7.5, 30°C Escherichia coli
2.7.7.18 0.11
-
 beta-nicotinate D-ribonucleotide wild-type, pH 7.5, 30°C Escherichia coli
2.7.7.18 1.7
-
 beta-nicotinate D-ribonucleotide mutant Y84V/Y118D, pH 7.5, 30°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Escherichia coli A0A140NE60 cf. EC 2.7.7.18
-
2.7.7.1 Escherichia coli BL21-DE3 A0A140NE60 cf. EC 2.7.7.18
-
2.7.7.18 Escherichia coli A0A140NE60
-
-
2.7.7.18 Escherichia coli BL21-DE3 A0A140NE60
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide very poor substrate for wild-type Escherichia coli diphosphate + NAD+
-
 ?
2.7.7.1 ATP + nicotinamide ribonucleotide very poor substrate for wild-type Escherichia coli BL21-DE3 diphosphate + NAD+
-
 ?
2.7.7.18 ATP + beta-nicotinate D-ribonucleotide
-
 Escherichia coli diphosphate + deamido-NAD+
-
 ?
2.7.7.18 ATP + beta-nicotinate D-ribonucleotide
-
 Escherichia coli BL21-DE3 diphosphate + deamido-NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.1 nadD
-
 Escherichia coli
2.7.7.18 nadD
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.1 0.76
-
 nicotinamide ribonucleotide wild-type, pH 7.5, 30°C Escherichia coli
2.7.7.1 3 6 nicotinamide ribonucleotide mutant A86W/Y118N, pH 7.5, 30°C Escherichia coli
2.7.7.1 6.6
-
 nicotinamide ribonucleotide mutant Y84V/Y118D, pH 7.5, 30°C Escherichia coli
2.7.7.18 1.5
-
 beta-nicotinate D-ribonucleotide mutant Y84V/Y118D, pH 7.5, 30°C Escherichia coli
2.7.7.18 4.9
-
 beta-nicotinate D-ribonucleotide wild-type, pH 7.5, 30°C Escherichia coli
2.7.7.18 25
-
 beta-nicotinate D-ribonucleotide mutant A86W/Y118N, pH 7.5, 30°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.1 0.08
-
 nicotinamide ribonucleotide wild-type, pH 7.5, 30°C Escherichia coli
2.7.7.1 8.3
-
 nicotinamide ribonucleotide mutant Y84V/Y118D, pH 7.5, 30°C Escherichia coli
2.7.7.1 48
-
 nicotinamide ribonucleotide mutant A86W/Y118N, pH 7.5, 30°C Escherichia coli
2.7.7.18 0.86
-
 beta-nicotinate D-ribonucleotide mutant Y84V/Y118D, pH 7.5, 30°C Escherichia coli
2.7.7.18 44
-
 beta-nicotinate D-ribonucleotide wild-type, pH 7.5, 30°C Escherichia coli
2.7.7.18 332
-
 beta-nicotinate D-ribonucleotide mutant A86W/Y118N, pH 7.5, 30°C Escherichia coli